Laboratory Introduction
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Crustaceans Virology and Immunology Lab
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Electron Microscopy Center in Institute of Marine Biology
Introduction
The Electron Microscopy (EM) Center in Institute of Marine Biology was established in 2001. The center offers the facilities and technical assistance to researchers to study the ultrastructure of biological samples. In the center, there are three microscope rooms and one preparing room for biological sample preparation. The facilities include a transmission EM, a scanning EM, and various indtruments for EM sample preparation.
Team Members
Director of the center: Dr. Jiann-Hong Leu, Associate Professor of Institute of Marine Biology
Manager: Mrs. Hsin-Yuan Chen, Administrative Executive Officer of Institute of Marine Biology
Instruments & services
Main Instruments:
1. Transmission Electron Microscope (TEM, Hitachi HT-7700)
2. Scanning Transmission Electron Microscope (STEM, Joel JEM 2010)
3. Scanning Electron Microscope (SEM, Hitachi S-2400)
4. Ultramicrotome (Leica EM UC6)
5. Critical Point Dryer (CPD, Hitachi HCP-2)
6. Ion Sputter (Hitachi E101)
7. Glass Knifemaker (Leica EM KMR2)
8. Light Compound Microscope (Olympus) & Dissecting Microscope (Olympus)
Services:
1. The various instruments are open for users who have been trained and qualified by the center
2. The center offers EM-related courses for undergraduate and graduate students and for training and qualifying new users to use the instruments
3. The center provides consultation for EM-related study, such as assisting users in developing appropriate methods and protocols.
4. The center provides staff-operated services, including biological sample preparation (negative stain and conventional resin embedding/sectioning) and instrument operation.
Research Results
A Anand, B Unnikrishnan, JY Mao, CJ Lin, JY Lai, CC Huang. 2022. Carbon-based low-pressure filtration membrane for the dynamic disruption of bacteria from contaminated water. Water Research 212, 118121.
A Sangili, B Unnikrishnan, A Nain, YJ Hsu, RS Wu, CC Huang, HT Chang. 2022. Stable carbon encapsulated titanium carbide MXene aqueous ink for fabricating high-performance supercapacitors. Energy Storage Materials 53, 51-61.
B Unnikrishnan, CW Wu, A Sangili, YJ Hsu, YT Tseng, JS Pandey, HT Chang, CC Huang. 2022. Synthesis and in situ sulfidation of molybdenum carbide MXene using fluorine-free etchant for electrocatalytic hydrogen evolution reactions. Journal of Colloid and Interface Science 628, 849-857.
CC Huang, CJ Lin, SY Chen, L Chang, HJ Lin. 2022. Curcumin carbon quantum dots and use thereof. US Patent 11,464,823.
CC Huang, JY Mao, CY Wang. Antitumor application of polysaccharide carbon nanogels. US Patent App. 17/666,538.
CJ Lin, B Unnikrishnan, CW Lehman, PH Wang, YJ Tseng, SG Harroun, SC Lin, CC Huang. 2022. Exploring molecular moieties on carbonized polymer dots from flavonoid glycosides with activity against enterovirus A71. Carbon 192, 285-294.
HC Chuang, TW Chu, AC Cheng, NY Chen, YS Lai. 2022. Iridovirus isolated from marine giant sea perch causes infection in freshwater ornamental fish. Aquaculture 548, 737588.
HH Chen, CJ Lin, A Anand, HJ Lin, HY Lin, JY Mao, PH Wang, YJ Tseng, WS Tzou, CC Huang, Robert YL Wang. 2022. Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein. Journal of Biological Chemistry 298 (6), 101957.
JY Mao, D Miscevic, B Unnikrishnan, HW Chu, CP Chou, L Chang, HJ Lin, CC Huang. 2022. Carbon nanogels exert multipronged attack on resistant bacteria and strongly constrain resistance evolution. Journal of Colloid and Interface Science 608, 1813-1826.
Oki Hayasaka, Hao-Yi Chang, Li-Chun Li, Yung-Che Tseng and Yi Ta Shao. 2022. Nocturnal Eyes in Slipper Lobsters (Decapoda Scyllaridae): Physiological and Ecological Implications of Habitat Depths for Visual Traits. Frontiers in Marine Science 9, 807347.
PH Lin, HJ Jian, YJ Li, YF Huang, A Anand, CC Huang, HJ Lin, JY Lai. 2022. Alleviation of dry eye syndrome with one dose of antioxidant, anti-inflammatory, and mucoadhesive lysine-carbonized nanogels. Acta Biomaterialia 141, 140-150.
RS Wu, YS Lin, A Nain, B Unnikrishnan, YF Lin, CR Yang, TH Chen, YF Huang, CC Huang, HT Chang. 2022. Evaluation of chemotherapeutic response in living cells using subcellular Organelle‒Selective amphipathic carbon dots. Biosensors and Bioelectronics, 114362.
WJ Lin, YS Lin, HT Chang, B Unnikrishnan, CC Huang. 2022. Electrocatalytic CuBr@ CuO nanoparticles based salivary glucose probes. Biosensors and Bioelectronics 194, 113610.
YF Lin, YS Lin, TY Huang, SC Wei, RS Wu, CC Huang, YF Huang, HT Chang. 2022. Photoswitchable carbon-dot liposomes mediate catalytic cascade reactions for amplified dynamic treatment of tumor cells. Journal of Colloid and Interface Science 628, 717-725.
YR Chiou, CJ Lin, SG Harroun, YR Chen, L Chang, AT Wu, FC Chang, YW Lin, HJ Lin, A Anand, B Unnikrishnan, A Nain, CC Huang. 2022. Aminoglycoside-mimicking carbonized polymer dots for bacteremia treatment. Nanoscale 14 (32), 11719-11730.
YS Lin, LW Chuang, YF Lin, SR Hu, CC Huang, YF Huang, HT Chang. 2022. Development of Fluorescent Carbon Nanoparticle-Based Probes for Intracellular pH and Hypochlorite Sensing. Chemosensors 10 (2), 64.
YS Lin, ZY Yang, A Anand, CC Huang, HT Chang. 2022. Carbon dots with polarity-tunable characteristics for the selective detection of sodium copper chlorophyllin and copper ions. Analytica Chimica Acta 1191, 339311.
Links
National Taiwan Ocean University